{"id":811,"date":"2025-08-27T10:05:06","date_gmt":"2025-08-27T13:05:06","guid":{"rendered":"http:\/\/172.16.1.60\/?page_id=811"},"modified":"2025-12-04T14:14:20","modified_gmt":"2025-12-04T18:14:20","slug":"microscopia-optica","status":"publish","type":"page","link":"http:\/\/cmabio.uea.edu.br\/index.php\/microscopia-optica\/","title":{"rendered":"Microscopia \u00d3ptica"},"content":{"rendered":"\n<div class=\"wp-block-stackable-columns alignfull stk-block-columns stk-block stk-51b2b12 stk-block-background stk--has-background-overlay\" data-block-id=\"51b2b12\"><style>.stk-51b2b12 .stk--block-align-51b2b12{align-items:center !important;}.stk-51b2b12 {background-color:#000000 !important;background-image:url(http:\/\/cmabio.uea.edu.br\/wp-content\/uploads\/2025\/11\/blog10.jpg) !important;background-position:bottom center !important;background-repeat:no-repeat !important;background-size:cover !important;padding-top:300px !important;padding-bottom:200px !important;margin-bottom:0px !important;}.stk-51b2b12:before{background-color:#000000 !important;opacity:0.6 !important;}.stk-51b2b12-column{--stk-column-gap:35px !important;row-gap:35px !important;}@media screen and (max-width:999px){.stk-51b2b12 {padding-top:250px !important;padding-bottom:150px !important;}}@media screen and (max-width:689px){.stk-51b2b12 {padding-top:150px !important;padding-bottom:100px !important;}}<\/style><div class=\"stk-row stk-inner-blocks stk--block-align-51b2b12 stk-block-content stk-content-align stk-51b2b12-column alignfull\">\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-0e2597c\" data-v=\"4\" data-block-id=\"0e2597c\"><style>.stk-0e2597c-container{margin-top:0px !important;margin-right:0px !important;margin-bottom:0px !important;margin-left:0px !important;}<\/style><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-0e2597c-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-0e2597c-inner-blocks\">\n<div class=\"wp-block-stackable-heading stk-block-heading stk-block-heading--v2 stk-block stk-45aa733\" id=\"microscopia-optica\" data-block-id=\"45aa733\"><style>.stk-45aa733 .stk-block-heading__text{color:var(--theme-palette-color-6, #F3F5F9) !important;}<\/style><h2 class=\"stk-block-heading__text has-text-color has-text-align-center\">Microscopia \u00d3ptica<\/h2><\/div>\n<\/div><\/div><\/div>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-spacer stk-block-spacer stk--no-padding stk-block stk-ccd096e\" data-block-id=\"ccd096e\"><style>.stk-ccd096e {height:140px !important;}<\/style><\/div>\n\n\n\n<div class=\"wp-block-stackable-heading stk-block-heading stk-block-heading--v2 stk-block stk-eee3fea\" id=\"o-que-e\" data-block-id=\"eee3fea\"><style>.stk-eee3fea .stk-block-heading__text{font-size:30px !important;font-weight:800 !important;font-family:system-ui, sans-serif !important;}@media screen and (max-width:999px){.stk-eee3fea .stk-block-heading__text{font-size:30px !important;}}<\/style><h2 class=\"stk-block-heading__text\">O que \u00e9?<\/h2><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-06df0e9\" data-block-id=\"06df0e9\"><p class=\"stk-block-text__text\">Um microsc\u00f3pio \u00f3ptico gera imagens a partir da radia\u00e7\u00e3o de ondas luminosas que s\u00e3o refratadas por um conjunto de lentes de vidro arranjadas a fim de minimizar aberra\u00e7\u00f5es crom\u00e1ticas, dessa forma, \u00e9 poss\u00edvel observar estruturas com aumentos m\u00e1ximos de 1500 a 2000 vezes, com resolu\u00e7\u00e3o limitada ao comprimento de onda da luz vis\u00edvel, <br>cerca de 200 nm.<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-4cd8027\" data-block-id=\"4cd8027\"><p class=\"stk-block-text__text\">Permite a visualiza\u00e7\u00e3o de amostras vivas por meio de m\u00e9todos que manipulam a luz para adicionar contraste, como contraste de fase e contraste de interfer\u00eancia diferencial (DIC), ou m\u00e9todos de fluoresc\u00eancia.<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-heading stk-block-heading stk-block-heading--v2 stk-block stk-1da6b59\" id=\"microscopio-confocal\" data-block-id=\"1da6b59\"><style>.stk-1da6b59 .stk-block-heading__text{font-size:30px !important;font-weight:800 !important;font-family:system-ui, sans-serif !important;}@media screen and (max-width:999px){.stk-1da6b59 .stk-block-heading__text{font-size:30px !important;}}<\/style><h2 class=\"stk-block-heading__text\">Microsc\u00f3pio Confocal<\/h2><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-9f4409c\" data-block-id=\"9f4409c\"><p class=\"stk-block-text__text\">O Microsc\u00f3pio Confocal \u00e9 utilizado para gerar imagens de alto contraste, tridimensionais e de alta defini\u00e7\u00e3o de amostras espessas, por meio da acopla\u00e7\u00e3o de um orif\u00edcio de abertura, chamado de pinhole, que est\u00e1 no mesmo plano focal do objeto de estudo, sendo o seu diferencial quando comparado \u00e0 microscopia de fluoresc\u00eancia convencional.<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-2f784d8\" data-block-id=\"2f784d8\"><p class=\"stk-block-text__text\">O microsc\u00f3pio confocal gera imagens por meio de sistemas eletr\u00f4nicos integrados, detectores e um sistemas de lasers combinados que varrem a superf\u00edcie da amostra ponto a a ponto, permitindo a visualiza\u00e7\u00e3o de c\u00e9lulas e tecidos vivos marcados com m\u00faltiplos fluor\u00f3foros.<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-spacer stk-block-spacer stk--no-padding stk-block stk-77b5a91\" data-block-id=\"77b5a91\"><style>.stk-77b5a91 {height:140px !important;}<\/style><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Microscopia \u00d3ptica O que \u00e9? Um microsc\u00f3pio \u00f3ptico gera imagens a partir da radia\u00e7\u00e3o de ondas luminosas que s\u00e3o refratadas por um conjunto de lentes de vidro arranjadas a fim de minimizar aberra\u00e7\u00f5es crom\u00e1ticas, dessa forma, \u00e9 poss\u00edvel observar estruturas com aumentos m\u00e1ximos de 1500 a 2000 vezes, com resolu\u00e7\u00e3o limitada ao comprimento de onda [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-811","page","type-page","status-publish","hentry"],"blocksy_meta":{"page_title_panel":"","has_hero_section":"disabled","fe1c64ab89945cda534f4bb2d579ae1a":"","hero_section":"type-1","hero_elements":[{"id":"custom_title","enabled":true,"heading_tag":"h1","title":"Home"},{"id":"custom_description","enabled":true,"description_visibility":{"desktop":true,"tablet":true,"mobile":false}},{"id":"custom_meta","enabled":false,"meta_elements":[{"id":"author","enabled":true,"label":"By","has_author_avatar":"yes","avatar_size":25},{"id":"post_date","enabled":true,"label":"On","date_format_source":"default","date_format":"M 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